Micronucleus test in fish and physicochemical parameters in water at Cocais dam, Minas Gerais
Abstract
The organisms known as bioindicators are used to assess changes in the aquatic environment. Incorporated chemical substances in natural environment interfere in the development of these organisms. The research had as objective to avaluate the water quality of the Cocais dam, in Patrocínio, Minas Gerais, using physical-chemical parameters of water and micronucleus test in fish. In this study were used fish of the species Oreochromis niloticus L. (Tilápia-do-nilo) introduced in the dam after its construction. Genetic material from twenty-eight individuals was collected at two points, P1 at the dam and P2 at the source. The cytological analysis was performed using an optical microscope and 4000 erythrocytes were examined per fish. For the physico-chemical characterization of the water samples were performed two collections during the rainy and dry periods, both in the dam and in the source. The parameters analyzed (turbidity, pH, Dissolved oxygen - OD, total dissolved solids – SDT), nitrate ammoniacal Nitrogen values were lower than those established in the legislation. The conductivity was higher than value indicated by CETESB, characterizing an indirect measure of the concentration of pollutants. Whit the results obtained in the micronucleus test a higher frequency of micronucleus can be observed in the cells of the fish captured in the dam, suggesting that the water is contaminated by genotoxics, that altered the genetic material of these bioindicators.
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References
Arslan, O. C.; Boyacioglu, M.; Parlak, H.; Katalay, S.; Karaaslan, M. A. 2015. Assessment of micronuclei induction in peripheral blood and gill cells of some fish species from Aliağa Bay Turkey. Marine Pollution Bulletin,94: 48-54.
Azevedo, S. M. C.; Vasconcelos, D. V.; Avelar, J. C. L. 2014. Análises físico-químicas do corpo hídrico na fazenda São Gonçalo em Paraty (RJ). Ibero‐Americana de Ciências Ambientais, 5(1).
BRASIL. Ministério do Meio Ambiente. Resolução CONAMA n° 357 de 17/03/2005. Alterada pela Resolução 410/2009 e pela 430/2011. Acessado em 19 de setembro de 2016.
CETESB. Companhia de Tecnologia de Saneamento Ambiental. Relatório de Qualidade das Águas Interiores do Estado de São Paulo. Série Relatórios. Apêndice A: Significado Ambiental e Sanitário das Variáveis de Qualidade das Águas e dos Sedimentos e Metodologias Analíticas e de Amostragem. 2009.
Contryman, P. I., Heddle, J. A. The production of micronuclei from chrosome aberrations in arradiated cultures of human lymphocytes. Mutation Research, v. 41, p. 321-332. 1976.
Correia, J. E.; Chistofoletti, C. A.; rodriguez, Y. A.; guedes, T.A.; fontanetti, C. S. 2017.Comet assay and micronucleus tests on Oreochromis niloticus (Perciforme: Cichlidae) exposed to raw sugarcane vinasse and to phisicochemical treated vinasse by pH adjustment with lime (CaO). Chemosphere, 173: 494-501.
Costa, D. D.; Kempka, A. P.; Skoronski, E. 2016. A contaminação de mananciais de abastecimento pelo nitrato: O panorama do problema no Brasil, suas consequências e as soluções potenciais. Revista Eletrônica do PRODEMA Fortaleza, Brasil, 10(2): 49-61.
Deutschmann, B,; Kolarevic, S.; Brack, W.; Kaisarevic, S.; Kostic, j. 2016. Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay B. Science of the Total Environment,.573: 1441–1449.
Fenech, M. 1993. The cytokinesis-blockmicronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations. Mutat. Res. v. 285, p. 35-44.
Júnior, E. O. C.; Pereir, B. B.; Morelli, S.; Pavanin, E. V.; Pavanin, L. A. 2016 Biological monitoring and B chromosomefrequency in Bagre (Rhamdia quelen) in southeast Brazil. Environmental Toxicology end Fharmacology, 38: 510-517.
Obiakor,, M. O.;Okonkwo, J. C.; Nnabude, P. C. Ezeonyejiaku, C. D. 2012. Eco-genotoxicology: Micronucleus Assay in Fish Erythrocytes as In situ Aquatic Pollution Biomarker: a Review. Journal of Animal Science Advances, 2(1): 123-133.
Parron, L. M.; Muniz, D. H. F.; Pereiea, C. M. 2011. Manual de procedimentos de amostragem e análise físico-química de água.
Pimenta, V. M. S. D.; Silva, J. M.; Nepomuceno, J. C.; Pavanin, L. P. 2013. In Situ Assessment of the Paraguay River Water, in Brazilian Pantanal, by Means of Micronucleus Assay with Fish and Chemical Analysis. Bull Environ Contam Toxicol, 90:427–433.
Ramsdorf, W. A.; Vicari, T.; Almeida, M. I. M.; Artoni, R. F.; Cestari, M. M. 2012. Handlingof Astyanax sp. for biomonitoring in Cangüiri Farm within a fountainhead (Iraí River Environment Preservation Area) through the use of genetic biomarkers. Environ Monit Assess, 184: 5841–5849.
Silva, E.; Fernanda. N. Costa, F. N.; Souza, T. L.; Viana, Z. C. V.; Souza, A. S.; Korn, M. G. A.; Ferreira, S.L.C. 2016. Assessment of Trace Elements in Tissues of Fish Species: Multivariate Study and Safety Evaluation, 27(12): 2234-2245.
Silva, S. V. S.; Dias, A. H. C.; Dutra, E. S.; Pavanin, A. L.; Morelli, S.; Pereira, B. B. 2015.The impact of water pollution on fish species in southeast region of Goiás, Brazil. Journal of Toxicology and Environmental Health, 79: 8-16.
Vasconcelos, M.G. Avaliação integrada da qualidade da água do Rio Uberabinha - MG com base na caracterização química dos sedimentos e de espécimes da ictiofauna. 2012. 188 f. Tese (Doutorado em Ciências Exatas e da Terra) - Universidade Federal de Uberlândia, Uberlândia, 2012. Acesso em: 15 de setembro de 2016.
Zalewska, T.; Danowska, B. 2017. Marine environment status assessment based on macrophytobenthic plants as bioindicators of heavy metals pollution. Marine Pollution Bulletin, 118: 281-288.