Toxicity of the hydroalcoholic extract of the leaves of Cissus sicyoides
Abstract
Cissus sicyoides L. (cipó-anil, vegetal insulin) belongs to the family Vitacea. It is much used by the population for the treatment of diabetes. The objective of this research was to perform a phytochemical screening of the hydroalcoholic extract of leaves of C. sicyoides to be used as primary source in the toxicological test with Artemia salina L. Were be phytochemical tests on the hydroalcoholic extract of the leaves of C. sicyoides for research on secondary metabolites and was evaluated the toxicological activity using A. saline cysts to quantify the mean lethal concentration (CL50). The extract presented steroids, tannins, flavonoids and saponins, compounds that had already been found in other studies of this species. It presented high toxicity to A. salina, reporting a Mean Lethal Concentration (CL50) of 930.7 g mL-1, serving as a parameter for the accomplishment of later studies in animal models with the pretension to reach more results for a safe use of C. sicyoides.
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References
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Silva, M.I.; Melo, C.T.; Vasconcelos, L.F.; Carvalho, A.M.; Sousa, F.C. 2012. Bioactivity and potential therapeutic benefits of some medicinal plants from the Caatinga (semi-arid) vegetation of Northeast Brazil: a review of the literature. Revista Brasileira de Farmacognosia, 22(1): 193-207.
Vasconcelos, T.H.C.D.; Modesto-Filho, J.; Diniz, M.F.F.M.; Santos, H.B.; Aguiar, F.B.D.; Moreira, P.V.L. 2007. Estudo toxicológico pré-clínico agudo com o extrato hidroalcoólico das folhas de Cissus sicyoides L. (Vitaceae). Revista Brasileira de Farmacognosia, 17(4): 583-591.
Xie, Y.H.; Deng, P.; Zhang, Y.Q.; Yu, W.S. 2009. Studies on the chemical constituents from Cissus assamica. Journal of Chinese medicinal materials, 32(2): 210-213.
Xu, F.; Matsuda, H.; Hata, H.; Sugawara, K.; Nakamura, S.; Yoshikawa, M. 2009. Structures of new flavonoids and benzofuran-type stilbene and degranulation inhibitors of rat basophilic leukemia cells from the Brazilian herbal medicine Cissus sicyoides. Chemical and Pharmaceutical Bulletin, 57(10): 1089-1095.
Ahmadu, A.A.; Onanuga, A.; Aquino, R. 2010. Flavonoid glycosides from the leaves of Cissus ibuensis hook (vitaceae). African Journal of Traditional, Complementary and Alternative Medicines, 7(3): 225-230.
Alcantara, R.G.L.; Joaquim, R.H.V.T.; Sampaio, S.F. 2016. Plantas medicinais: o conhecimento e uso popular. Revista de APS, 18(4): 470-482.
Allen Jr, L.V. 2015. Introdução à Farmácia de Remington. Artmed Editora, Porto Alegre, p. 9-26.
Arcanjo, D.D.R.; Albuquerque, A.C.M.; Melo-Neto, B.; Santana, L.C.L.R.; Medeiros, M.; Citó, A.M.G.L. 2012.
Bioactivity evaluation against Artemia salina Leach of medicinal plants used in Brazilian Northeastern folk medicine. Brazilian Journal of Biology, 72(3): 505-509.
Balbinot, S.; Velasquez, P.G.; Düsman, E. 2013. Reconhecimento e uso de plantas medicinais pelos idosos do Município de Marmeleiro–Paraná. Revista Brasileira de Plantas Medicinais, 15(4), 632-638.
Banu, J. 2012. Medicinal properties of plants from the genus Cissus: A review. Journal of Medicinal Plants Research, 6(16): 3080-3086.
Beltrame, F.; Ferreira, A.; Cortez, D. 2002. Coumarin glycoside from Cissus sicyoides. Natural product letters, 16(4): 213-216.
Beserra, F.P.; Santos, R.C.; Périco, L.L.; Rodrigues, V.P.; Kiguti, L. R.A.; Saldanha, L.L.; ... Hiruma-Lima, C. A. 2016. Cissus sicyoides: Pharmacological Mechanisms Involved in the Anti-Inflammatory and Antidiarrheal Activities. International journal of molecular sciences, 17(2): 149.
Brasileiro, B.G.; Pizziolo, V.R.; Raslan, D.S.; Jamal, C.M.; Silveira, D. 2006. Antimicrobial and cytotoxic activities screening of some Brazilian medicinal plants used in Governador Valadares district. Revista Brasileira de Ciências Farmacêuticas, 42(2): 195-202.
Khan, M.A.; Nabi, S.G.; Prakash, S.; Zaman, A. 1986. Pallidol, a resveratrol dimer from Cissus pallida. Phytochemistry, 25(8): 1945-1948.
Klaassen, C.D.; Watkins III, J.B. Fundamentos em toxicologia de Casarett e Doul. 2. ed. Porto Alegre: AMGH, 2012. 472p.
Lans, C.A. 2006. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus. Journal of ethnobiology and ethnomedicine, 2(1): 45.
Mclaughlin, J.L.; Rogers, L.L.; Anderson, J.E. 1998. The use of biological assays to evaluate botanicals. Drug information journal, 32(2): 513-524.
Meyer, B.N.; Ferrigni, N.R.; Putnam, J.E.; Jacobsen, L.B.; Nichols, D.J.; McLaughlin, J.L. 1982. Brine shrimp: a convenient general bioassay for active plant constituents. Planta medica, 45(05): 31-34.
Messias, M.C.T.B.; Menegatto, M.F.; Prado, A.C.C.; Santos, B.R.; Guimarães, M.F.M. 2015. Popular use of medicinal plants and the socioeconomic profile of the users: a study in the urban area of Ouro Preto, Minas Gerais, Brazil. Revista Brasileira de Plantas Medicinais, 17(1): 76-104.
Omale, J.; Okafor, P.N. 2008. Comparative antioxidant capacity, membrane stabilization, polyphenol composition and cytotoxicity of the leaf and stem of Cissus multistriata. African Journal of Biotechnology, 7(17): 3129-3133.
Parra, A.L.; Yhebra, R.S.; Sardiñas, I.G.; Buela, L.I. 2001. Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts. Phytomedicine, 8(5): 395-400.
Rashid, R.B.; Towsif, F.N.; Bushra, F.A.; Tahia, F. 2016. Antioxidant, Membrane Stabilizing and Cytotoxic Activities of Cissus adnata (Roxb.). Dhaka University Journal of Pharmaceutical Sciences, 15(1): 69-71.
Rosa, C.; Veras, K.; Silva, P.; Lopes Neto, J. J.; Cardoso, H.; Alves, L.; ... MORAES, D. 2016. Composição química e toxicidade frente Aedes aegypti L. e Artemia salina Leach do óleo essencial das folhas de Myrcia sylvatica (G. Mey.) DC. Revista Brasileira de Plantas Medicinais, 18(1): 19-26.
Saifah, E.; Kelley, C.J.; Leary, J.D. 1983. Constituents of the leaves of Cissus rheifolia. Journal of natural products, 46(3): 353-358.
Saleh, Z.; Zuhud, E.A.M.; Sari, R.K. 2015. Phytochemical screening and antioxidant activity of ethanolic extract of Rhizanthes deceptor (Rafflesiaceae) and its host Tetrastigma papillosum. Research Journal of Medicinal Plant, 9(6): 293-299.
Sharma, C.; Rajendar, K.; Kumari, T.; Arya, K.R. 2014. Indian traditional therapies and bio-prospecting: their role in drug development research. International Journal of Pharmaceutical Sciences and Research, 5(3): 730-741.
Silva, G.A.D.; Araújo, L.C.; Oga, S.; Akisue, G. 1996. Estudo toxicológico e farmacológico dos extratos fluídos de Cissus sicyoides L. Revista Brasileira de Farmacognosia, 5(2): 143-155.
Silva, M.I.; Melo, C.T.; Vasconcelos, L.F.; Carvalho, A.M.; Sousa, F.C. 2012. Bioactivity and potential therapeutic benefits of some medicinal plants from the Caatinga (semi-arid) vegetation of Northeast Brazil: a review of the literature. Revista Brasileira de Farmacognosia, 22(1): 193-207.
Vasconcelos, T.H.C.D.; Modesto-Filho, J.; Diniz, M.F.F.M.; Santos, H.B.; Aguiar, F.B.D.; Moreira, P.V.L. 2007. Estudo toxicológico pré-clínico agudo com o extrato hidroalcoólico das folhas de Cissus sicyoides L. (Vitaceae). Revista Brasileira de Farmacognosia, 17(4): 583-591.
Xie, Y.H.; Deng, P.; Zhang, Y.Q.; Yu, W.S. 2009. Studies on the chemical constituents from Cissus assamica. Journal of Chinese medicinal materials, 32(2): 210-213.
Xu, F.; Matsuda, H.; Hata, H.; Sugawara, K.; Nakamura, S.; Yoshikawa, M. 2009. Structures of new flavonoids and benzofuran-type stilbene and degranulation inhibitors of rat basophilic leukemia cells from the Brazilian herbal medicine Cissus sicyoides. Chemical and Pharmaceutical Bulletin, 57(10): 1089-1095.
Published
2017-01-15
How to Cite
DIAS, Gabriela Tafaela et al.
Toxicity of the hydroalcoholic extract of the leaves of Cissus sicyoides.
Acta Brasiliensis, [S.l.], v. 1, n. 1, p. 8-12, jan. 2017.
ISSN 2526-4338.
Available at: <http://revistas.ufcg.edu.br/actabra/index.php/actabra/article/view/8>. Date accessed: 24 dec. 2024.
doi: https://doi.org/10.22571/Actabra1120178.
Section
Pharmacy
