Evaluation of volatile constituents, exudation of resin and occurrence of galls of Protium aracouchini (Aubl.) Marchand
Abstract
Protium aracouchini (Aubl.) Marchand [sin Icica aracouchini Aubl.], which occurs in the Adolpho Ducke Forest Reserve, in the Brazilian Amazon, was evaluated for the presence of galls, for resin exudation and the composition of the essential oils from the aerial parts and the resin. The experiment to stimulate the exudation of resin from the trunk was conducted using a 2-chloroethylphosphonic acid solution. The resin produced after 40 days and the aerial parts had their essential oils extracted in a Clevenger apparatus and the volatile chemical constituents were analyzed using GC/MS. The non-oxygenated sesquiterpenes α-copaene (21.15%) and α-gurjunene (13.69%), in addition to the oxygenated sesquiterpene spathulenol (10.32%), were detected as the majority constituents of the essential oil of the leaves, and a concentration similar to that of α-gurjunene was found in the branches (13.28%). The resin essential oil showed a high concentration of hydrocarbon monoterpenes (76.49%) with a predominance of α-pinene (17.57%) and limonene (46.11%). Four gall morphotypes were found associated with this species. The present study reports for the first time information on the volatile constituents and the resinous potential of P. aracouchini, and registers the morphotypes of the galls that help in the taxonomy of the species.
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References
Carvalho, L. E., Pinto, D. S., Magalhães, L. A. M., Lima, M. P., Marques, M. O. M., & Facanali, R. (2010). Chemical constituents of essential oil of Protium decandrum (Burseraceae) from Western Amazon. Journal of Essential Oil Bearing Plants, 13(2), 181-184. doi: 10.1080/0972060X.2010.10643809
Carvalho, F. S. P. (2010). Insetos galhadores associados à família Burseraceae da reserva florestal Ducke, Manaus, Amazonas, Brasil. (Dissertação de Mestrado). Instituto Nacional de Pesquisas da Amazônia. Manaus, Amazonas.
Daly, C. D. (1992). New taxa and combinations in Protium Burm. f. Studies in neotropical Burseraceae VI. Brittonia, 44(3), 280-299. doi: https://doi.org/10.2307/2806927.
Fernandes, G. W. A., Tameirão-Neto, E., & Martins, R. G. (1988). Ocorrência e caracterização de galhas entomógenas na vegetação do Campus Pampulha da Universidade Federal de Minas Gerais. Revista Brasileira de Zoologia, 5(1), 11-29. doi: 10.1590/S0101-81751988000100002.
Freitas, J. G. R., Camara, C. A. G., Moraes, M. M., & Silva, H. C. H. Volatile constituents of two species of Protium from the atlantic rainforest in the state of Pernambuco, Brazil. (2011). Natural Product Communications, 6(11), 1727-1730. doi: 10.1177/1934578X1100601139
Lima, T. A. A. C., Rocha, K. R. A., Melo, M. F. F., Marques, M. O. M., Facanali, R., & Lima, M. P. (2014). Caracterização morfológica e análise de voláteis da espécie resinífera Protium spruceanum (Benth.) Engl. do campus do INPA, Manaus-AM. Scientia Amazonia, 3(2), 6-10.
Lima, T. A. A. C., Ribeiro, J. E. L. S., Marques, M. O. M., Facanali, R., & Lima, M. P. (2016). Estimulo para produção de resina em Protium hebetatum Daly e avaliação dos constituintes químicos voláteis. Scientia Amazonia, 5(3), 21-24.
Mclafferty, F. W., & Stauffer, D. (1989). The Wiley/NBS Registry of Mass Spectral Data. New York: Wiley –Interscience Pub.
Pinto, D. S., Carvalho, L. E., Lima, M. P., Marques, M. O. M., Facanali, R., & Ribeiro, J. E. L. S. (2010). Volatiles of foliar rachis, branches and resin elicited by insects from Protium hebetatum grows wild in Amazon. Journal of Essential Oil Bearing Plants, 13(6), 699-703. doi: 10.1080/0972060X.2010.10643881.
Ramos M. F. S., Siani, A. C., Tappin, M. R. R., Guimarães, A. C., & Ribeiro, J. E. L. S. (2000). Essential oils from oleoresins of Protium spp. of the Amazon region. Flavour and Fragrance Journal, 15(6), 383-387. doi: 10.1002/1099-1026(200011/12)15:6<383::AID-FFJ927>3.0.CO;2-X
Ribeiro, J. E. S., Hopkins, M. J. G., Vicentini, A., Sothers, C. A., Costa, M. A. S., Brito, J. M., Souza, M. A. D., Martins, L. H. P., Lohmann, L. G., Assunção, P. C. L., Pereira, E. C., Silva, C. F., Mesquita, M, R., & Procópio, L. C. (1999). Flora da Reserva Ducke: Guia de Identificação das Plantas Vasculares de uma Floresta de Terra-firme na Amazônia Central. Manaus: INPA/DFID (Eds.), Manaus-Amazonas.
Santana, R. C., Santos Rosa, A. S., Mateus, M. H. S., Soares, D. C., Atella, G., Guimarães, A. C., Carlos Siani, A. C., Ramos, M. F. S., Saraiva, E. M., & Silva, L. H. P. (2020). In vitro leishmanicidal activity of monoterpenes present in two species of Protium (Burseraceae) on Leishmania amazonenses. Journal of Ethnopharmacology, 15, 112981. doi: 10.1016/j.jep.2020.112981.
Souza, L. R., Trindade, F. G., Oliveira, R. A., Costa, L. C. B., Gomes, V. M., & Cunha, M. (2016). Histochemical characterization of secretory ducts and essential oil analysis of Protium species (Burseraceae). Journal of Essential Oil Research, 28(2), 166-171. doi: 10.1080/10412905.2015.1092478
Zoghbi, M. G. B., Andrade, E. H. A., Lima, M. P., Silva, T. M. D., & Daly, D. C. (2005). The essential oils of five species of Protium growing in the north of Brazil. Journal of Essential Oil Bearing Plants, 8(3), 312-317. doi: 10.1080/ 0972060X. 2005.10643458.
