Secondary metabolites and antioxidant action of Croton heliotripifolius and Croton blanchetianus

DOI: https://doi.org/10.22571/Actabra13201730

Abstract

Antioxidants are compounds that, at optimal concentrations in relation to oxidable substrates, react with free radicals preventing or reducing oxidative stress. Many diseases and degenerative processes are associated with overproduction of free radicals. This stimulated several research groups to investigate the antioxidant potential of substances produced by several families of the world flora. This study identified the major classes of secondary metabolites present in ethanol leaf extracts of C. blanchetianus (Marmeleiro) and C. helliotripiifolius (Velame), and assessed their antioxidant potential. Ethanol extracts were obtained by the exhaustive extraction method cold and resulted in 3.29% and 3.26% extraction efficiency. Then phytochemical analyses were performed on the extracts to investigate the presence of tannins, flavonoids, flavonones, flavonols, catechins and xanthones. The antioxidant activity of extracts was determined by in vitro photocolorimetric method based on the sequestration of free radicals, using DPPH (1,1-diphenyl-2-picryl-hydrazyl). Both extracts were able to sequestrate DPPH free radical. Croton blanchetianus extract activity showed to be more efficient than BHT (Butylated Hidroxytoluene) (positive control), with EC50 = 6.5 ± 0.5 mg mL-1. Croton helliotripifolius extracts showed lower antioxidant activity, with EC50 = 50.15 ± 0.21 but still higher than that observed for BHT. However, the results observed for the ethanol extracts are possibly the result of the presence and concentration of phenolic compounds such as tannins and flavonoids, which were identified in this study.

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References

Venancio, A.M. 2006. Toxicidade aguda e atividade antinociceptiva do óleo essencial do Ocimum basilicum L. (manjericão), em Mus músculos (camundongos). Dissertação de Mestrado, Universidade Federal de Sergipe, Aracaju. 110f
Bakkali, F.; Averbeck, S.; Averbeck, D.; Idaomar, M. 2008. Biological effects of essentials oils: A review. Food and Chemical Toxicology. 46: 446-475.
Basile, A.; Ferrara, L.; Del Pozzo, M., Mele, G.; Sorbo, S.; Bassi, P.; Montesano, D. 2007. Antibacterial and antioxidant activities of ethanol extract from Paullinia cupana Mart. Journal of Ethnopharmacology. 102: 32-36.
Boudet, A.M. 2007. Evolution and current status of research in phenolic compounds. Phytochemistry. 68: 22-24.
Balestrin, L.; Dias, J.F.G.; Miguel, O.G.; Dall’Stella, D.S.G.; Miguel, M.D. 2008. Contribuição ao estudo fitoquímico de Dorstenia multiformis Miquel (Moraceae) com abordagem em atividade antioxidante. Revista Brasileira de Farmacognosia. 18: 230-235.
Catunda-Junior, F.E.A.; Luciano, J.H.S.; Morais, S.M. 2002. Atividade antioxidante de óleos essenciais de plantas do Nordeste do Brasil. Revista de Ciência & Tecnologia, 4:. 23-29.
EL-Remessy, A.B.; Behzadian, M.A.; Abou-Mohamed, G.; Franklin, T.; Caldwell, R.W.; Caldwell, R.B. 2003. Experimental diabetes causes breakdown of the blood-retina barrier mechanism involving tyrosine nitration and increases in expression of vascular endothelial growth factor and urokinase plasminogen activator receptor. American Journal of Pathology, v.162, p. 1995-2004.
Fonseca, A.M.; Bizerra, A.M.C.; Souza, J.S.N.; Monte, F.J.Q.; Oliveira, M.C.F.; Mattos, M.C.; Cordel, G.A.; Braz-Filho, R.; Lemos, T.L.G. 2009. Constituents and antioxidant activity of two varieties of coconut water (Cocos nucifera L.). Revista Brasileira de Farmacognosia, 19:193-198.
Matos, F.J.A. 1997. Introdução à fitoquímica experimental, 1 ed. UFC,. 141p.
Mensor, L.L.; Menezes, F.S.; Leitão, G.G.; Reis, A.S.; Dos Santos, T.C.; Coube, C.S.; Leitão, S.G. 2001. Screnning of Brasilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Research, 15: 127-130.
Morais, S.M.; Catunda-Junior, F.E.A.; Silva, A.R.A.; Stone, J.; Neto, M. 2006. Atividade antioxidante de óleos essenciais de espécies de Croton do nordeste do Brasil. Química Nova, 29: 907-910.
Melo, E.A.; Maciel, M.I.S.; Lima, V.L.; Nascimento, R.J. 2008. Capacidade antioxidante de frutas. Revista Brasileira de Ciências Farmacêuticas. 44: 193-201.
Melo, J.B.; Araújo, T.A.S.; Castro. V.T.N.A.; Cabral, D.L.V.; Rodrigues, M.D.; Nascimento, S.C.; Amorin, E.L.C.; Alburquerque, U.L. 2010. Antiproliferative Activity, Antioxidant Capacity and Tannin Content in Plants of Semi-Arid Northeastern Brazil. Molecules. 15, 8534-8542.
Nascimento, P. F.; Rodrigues, C. S. 2007. Atividade antimicrobiana dos óleos essenciais: uma abordagem multifatorial dos métodos. Brazilian Journal of Pharmacognosy. 17: 305-311.
Nardi, G.M.; Felippi, R.; Dalbos, S.; Siqueira-Junior, J.M.; Arruda, D.C.; Delle-Monache, F. 2003. Anti-inflammatory and antioxidant effects of Croton celtidifolius bark. Phytomedicine. 10: 176–184.
Nunes, X.P.; Mesquita, R.F.; Silva, D.A.; Lira, D.P.; Costa, V.C.O.; Silva, M.V.B.; Xavier, A.L., Diniz, M.F.F.M.; Agra, M.F. 2008. Constituintes químicos, avaliação das atividades citotóxica e antioxidante de Mimosa paraibana Barneby (Mimosaceae). Revista Brasileira de Farmacognosia. 18: 718-723.
Pimentel, F.O. 2006. Atividade antioxidante de Byrsonima crassa Nied. e Byrsonima fagifolia Nied. em modelos de indução de úlcera gástrica.. Dissertação de Mestrado em Farmacologia. Universidade Estadual de Campinas, SP. 111p.
Randau, K.P. 2004. Estudo Farmacognóstico de Croton rhamnifolius H.B.K. e Croton rhamnifolioides pax & hoffm. (Euphorbiaceae). Revista Brasileira de Farmacognosia. 14: 89-96.
Razavi, S.M.; Nazemiyeh, H.; Hajiboland, R.; Kumarasamy, Y.; Delazar, A.; Nahar, L.; Saker, S.D. 2008. Coumarins from the aerial parts of Prangos uloptera (Apiaceae). Revista Brasileira de Farmacognosia. 18: 1-5.
Rebelo, M.M.; Silva, J.K.R.; Aandrade, E.H.A.; Maia, J.G.S. 2009. Antioxidant capacity and biological activity of essential oil and methanol extract of Hyptis crenata (Pohl) ex Benth. Revista Brasileira de Farmacognosia. 19: 230-235.
Sousa, C.M.; Silva, H.R.E.; Vieira-Junior, G.M.; Ayres, M.C.C.; Costa, C.L.S., Araujo, D.S.; Cavalcante, L.C.D.; Barros, E.D.S.; Araújo, P.B.M.; Brandão, M.S.; Chaves, M.H. 2007. Fenóis totais e atividade antioxidante de cinco plantas medicinais. Química Nova. 30: 351-355.
Souza, T.J.T.; Apel, M.A.; Bordignon, S.; Matzenbacher, N.I.; Zuanazzi, J.A.S.; Henriques, A.T. 2007. Composição química e atividade antioxidante do óleo volátil de Eupatorium polystachyum DC. Revista Brasileira de Farmacognosia. 17: 368-372.
Sikora, E.; Cieslik, E.; Leszczynska, T.; Filipiak-Florkiwuacz, A.; Pilsulewski, P.M. The antioxidant activity of selected cruciferous vegetables subjected to aquathermal processing. Food Chemistry. 107: 50-55, 2008.
Van Den Berg, R.; Haenen, G.; Van Den Berg, H.; Vander, V.W.; Bast, A. 2000. The predictive value of the antioxidant capacity of structurally related flavonoids using the trolox equivalent antioxidant capacity (TEAC) assay. Food Chemistry. 703: 391-395.
Published
2017-09-26
How to Cite
AQUINO, Vitória Viviane Ferreira et al. Secondary metabolites and antioxidant action of Croton heliotripifolius and Croton blanchetianus. Acta Brasiliensis, [S.l.], v. 1, n. 3, p. 28-31, sep. 2017. ISSN 2526-4338. Available at: <http://revistas.ufcg.edu.br/actabra/index.php/actabra/article/view/30>. Date accessed: 22 nov. 2024. doi: https://doi.org/10.22571/Actabra13201730.
Citation Formats
Issue
Vol 1 No 3 (2017): Acta Brasiliensis
Section
Pharmacy