Identificação de algumas espécies amazônicas de Culex (Culex) e Culex (Melanoconion) por meio de morfotipagem e barcoding
Abstract
Os mosquitos Culex spp. apresentam características idiossincráticas e sua baixa variabilidade dificulta sua identificação. O objetivo do nosso estudo foi analisar a região 5 'do gene da subunidade I do citocromo oxidase (coI) para a identificação taxonômica de espécies de Culex que foram previamente diagnosticadas em subgêneros Culex e Melanoconion em condições de campo. Dez sequências de espécimes foram analisadas pelo Automatic Barcode Gap Discovery (ABGD). Todas as sequências apresentaram 94-99% de identidade quando comparadas com outras sequências de espécies de Culex disponíveis no GenBank. Cinco partições iniciais suportaram 80-88 grupos de espécies. Entre eles, oito conjuntos continham os espécimes do presente estudo. Das 10 sequências de mosquitos, cinco não formaram nenhum cluster consistente, e as demais apresentaram alguma consistência no diagnóstico taxonômico nas condições de campo. Nossos resultados sugerem que algumas sequências do gene coI de espécimes podem pertencer a espécies do subgênero Melanoconion, cuja sequência 5' coI é desconhecida ou inédita no GenBank.
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References
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Collins, R.A., & Cruickshank, R.H. (2014). Known Knowns, Known Unknowns, Unknown Unknowns and Unknown Knowns in DNA Barcoding: A Comment on Dowton et al. Systematic Biology, 63, 1005-1009. doi: http://doi.org/10.1093/sysbio/syu060
Consoli, R.A.G.B., & Lourenço-de-Oliveira, R. (1994). Principais mosquitos de importância sanitária no Brasil. Cadernos de Saúde Pública. Riode Janeiro: Fiocruz. Recovered from http://www.scielo.br/pdf/csp/v11n1/v11n1a19.pdf
Demari-Silva, B., Vesgueiro, F.T., Sallum, M.A., & Marrelli, M.T. (2011). Taxonomic and phylogenetic relationships between species of the genus Culex (Diptera: Culicidae) from Brazil inferred from the cytochrome c oxidase I mitochondrial gene. Journal of Medical Entomology, 48, 272–279. doi: http://dx.doi.org/10.1603/ME09293
Dibo, M.R., Menezes, R.M.T., Ghirardelli, C.P. Mendonça, A.L., & Chiaravalloti Neto, F. (2011). Presença de culicídeos em município de porte médio do Estado de São Paulo e risco de ocorrência de febre do Nilo Ocidental e outras arboviroses. Revista da Sociedade Brasileira de Medicina Tropical, 44, 496-503. http://dx.doi.org/10.1590/S0037-86822011000400019
Eldridge, B.F. (2005). Mosquitoes, the Culicidae. In: Marquardt WH (ed) Biology of disease vectors. 2nd edn. Chapter 9, Elsevier Academic, Burlington, pp 95-111. 2005. doi: http://dx.doi.org/10.3201/eid1108.050610
Ferreira-de-Brito, A., Ribeiro, I.P., Miranda, R.M., Fernandes, R.S., Campos, S.S., Silva, K.A.B., Castro, M.G., Bonaldo, M.C., Brasil, P., Lourenço-de-Oliveira, R., Ferreira-de-Brito, A., Ribeiro, I.P., Miranda, R.M., Fernandes, R.S., Campos, S.S., Silva, K.A.B., Castro, M.G., Bonaldo, M.C., & Brasil, P. (2016). First detection of natural infection of Aedes aegypti with Zika virus in Brazil and throughout South America. Memórias do Instituto Oswaldo Cruz, 111, 655-658. doi: http://doi.org/10.1590/0074-02760160332
Forattini, O.P. (2002). Culicidologia médica, vol 2: Identificação, Biologia, Epidemiologia. São Paulo - Editora da Universidade de São Paulo.
Gomes, A.C., Natal, D., Paula, M.B., Urbinatti, P.R., Mucci, L.F., & Bitencourt, M.D. (2007). Riqueza e abundância de Culicidae (Diptera) em área impactada, Mato Grosso do Sul, Brasil. Revista de Saúde Pública, 41, 661-664. http://dx.doi.org/10.1590/S0034-89102007000400023
Gutierrez, C.T. (2015). The subgenus Melanoconion of Culex in South America ( Diptera : Culicidae). Universidade de São Paulo - Faculdade de Saúde Pública (Dissertação). 2015.doi: http://dx.doi.org/10.11606/T.6.2015.tde-18052015-102016
Hall, T. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series. http://brownlab.mbio.ncsu.edu/jwb/papers/1999hall1.pdf
Harbach, R.E. (2011). Classification within the cosmopolitan genus Culex (Diptera: Culicidae): The foundation for molecular systematics and phylogenetic research. Acta Tropica, 120, 1-14. doi: http://doi.org/10.1016/j.actatropica.2011.06.005
Harbach, R.E. (2012). Culex pipiens: species versus species complex taxonomic history and perspective. Journal of the American Mosquito Control Association, 28 (4 Suppl.): 10-23. doi: http://doi.org/10.2987/8756-971X-28.4.10
Harbach, R.E. (2013). Mosquito Taxonomic Inventory. Recovered from http://www.mosquito-taxonomic-inventory.info/.
Hebert, P.D.N., Cywinska, A., Ball, S.L., & deWaard, J.R. (2003). Biological identifications through DNA barcodes. Proceedings. Biological sciences / The Royal Society, 270, 313-321. doi: http://doi.org/10.1098/rspb.2002.2218
Hebert, P.D.N., Penton, E.H., Burns, J.M., Janzen, D.H., & Hallwachs, W. (2004). Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences of the United States of America, 101, 14812-14817. doi: https://doi.org/10.1073/pnas.0406166101
Hernández-Triana, L.M., Brugman, V.A., Nikolova, N.I., Ruiz-Arrondo, I., Barrero, E., Thorne, T., Marco, M.F., Krüger, A., Lumley, S., Johnson, N., & Fooks, A.R. (2019). DNA barcoding of British mosquitoes (Diptera, Culicidae) to support species identification, discovery of cryptic genetic diversity and monitoring invasive species. ZooKeys 832, 57-76. doi: https://doi.org/10.3897/zookeys.832.32257
Hoyos-López, R., Suaza-Vasco, J., Rúa-Uribe, G., Uribe, S., & Gallego-Gómez, J.C. (2016). Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean. Memórias do Instituto Oswaldo Cruz, 111, 625-634. doi: http://dx.doi.org/10.1590/0074-02760160096
Hutchings, R.S.G., Sallum, M.A.M., & Hutchings, R.W. (2011). Mosquito (Diptera: Culicidae) diversity of a forest-fragment mosaic in the Amazon rain forest. Journal of Medical Entomology, 48, 173-187. doi: https://dx.doi.org/10.1603/me10061
Kimura, M. (1980). A Simple Method for Estimating Evolutionary Rates of Base Substitutions Through Comparative Studies of Nucleotide Sequences. Journal Molecular Evolution, 16, 111-120. doi: http://dx.doi.org/10.1007/bf01731581
Kobayashi, K.M. (1999). Revisão das espécies do grupo Atratus de Culex (Melanoconion) (Diptera: Culicidae). Tese de Doutorado. Universidade de São Paulo. Faculdade de Saúde Pública.
Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870-1874. http://doi.org/10.1093/molbev/msw054
Lane, J. (1953). Neotropical Culicidae. vol. 2. São Paulo, Press in University of São Paulo.
Laurito, M., Oliveira, T.M.P., Almirón, W.R., & Sallum, M.A.M. (2013). coI barcode versus morphological identification of Culex (Culex) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil. Memórias do Instituto Oswaldo Cruz, 108, 110-122.doi: http://dx.doi.org/10.1590/0074-0276130457
Louise, C., Vidal, P.O., & Suesdek, L. (2015). Microevolution of Aedes aegypti. PLoS ONE 10(9): e0137851. doi: http://dx.doi.org/10.1371/journal.pone.0137851
Pugedo, H., Barata, R.A., França-Silva, J.C., Silva, J.C., & Dias, E.S. (2005). HP: um modelo aprimorado de armadilha luminosa de sucção para a captura de pequenos insetos. Revista da Sociedade Brasileira de Medicina Tropical, 38, 70-72. doi: http://dx.doi.org/10.1590/S0037-86822005000100017
Puillandre, N., Lambert, A., Brouillet, S., & Achaz, G. (2012). ABGD, Automatic Barcode Gap Discovery for primary species delimitation. Molecular Ecology, 21, 1864-1877. doi: http://doi.org/10.1111/j.1365-294X.2011.05239.x
Ratnasingham, S., & Hebert, P.D.N. (2007). BARCODING, BOLD : The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes, 7, 355-364. doi: http://dx.doi.org/10.1111/j.1471-8286.2007.01678.x.
Sallum, M.A.M. (1994). Revisão da Seção Spissipes de Culex (Melanoconion) (Diptera: Culicidae). Recovered from http://www.teses.usp.br/teses/disponiveis/6/6132/tde-15072016-150054/publico/DR_243_Sallum_1994.pdf
Sallum, M.A.M., & Forattini, O.P. (1996). Revision of the Spissipes Section of Culex (Melanoconion) (Dipeta: Culicidae), 12(3):517-600. Recovered from http://www.teses.usp.br/teses/disponiveis/6/6132/tde-15072016 150054/publico/DR_243_Sallum_1994.pdf
Sirivanakarn, S. (1982). A Review of the Systematics and a Proposed Scheme of Internal Classification of the New World Subgenus Melanoconion of Culex. Mosquito Systematics, 14. http://mosquito-taxonomic-inventory.info/sites/mosquito-taxonomic-inventory.info/files/Sirivanakarn%201983.pdf
Song, S., Li, Y., Fu, S., Liu, H., Li, X., Gao, X., Xu, Z., Liu, G., Wang, D., Tian, Z., Zhou, J., He, Y., Lei, W., Wang, H., Wang, B., Lu, X., & Liang, G. (2017). Could Zika virus emerge in Mainland China? Virus isolation from nature in Culex quinquefasciatus, 2016. Emerging Microbes & Infections, 6, e93. doi: http://doi.org/10.1038/emi.2017.80
Sumruayphol, S., Apiwathnasorn, C., Ruangsittichai, J., Sriwichai, P., Attrapadung, S., Samung, Y., & Dujardin, J.P. (2016). DNA barcoding and wing morphometrics to distinguish three Aedes vectors in Thailand. Acta Tropica, 159, 1-10.doi: http://doi.org/10.1016/j.actatropica.2016.03.010
Torres-Gutierrez, C., Bergo, E.S., Emerson, K.J., de Oliveira, T.M.P., Greni, S., Sallum, M.A.M. (2016). Mitochondrial coI gene as a tool in the taxonomy of mosquitoes Culex subgenus Melanoconion. Acta Tropica, 164, 137-149. doi: http://doi.org/10.1016/j.actatropica.2016.09.007
Viana, L.A., Soares, P., Paiva, F., & Lourenço-de-Oliveira, R. (2010). Caiman-Biting Mosquitoes and the Natural Vectors of Hepatozoon caimani in Brazil. Journal of Medical Entomology, 47, 670-676. doi: http://doi.org/10.1603/me09260
Williams, M.R., & Savage, H.M. (2009). Identification of Culex (Melanoconion) species of the United States using female cibarial armature (Diptera: Culicidae). Journal of Medical Entomology, 46, 745-752. doi: http://doi.org/10.1603/033.046.0404
Collins, R.A., & Cruickshank, R.H. (2014). Known Knowns, Known Unknowns, Unknown Unknowns and Unknown Knowns in DNA Barcoding: A Comment on Dowton et al. Systematic Biology, 63, 1005-1009. doi: http://doi.org/10.1093/sysbio/syu060
Consoli, R.A.G.B., & Lourenço-de-Oliveira, R. (1994). Principais mosquitos de importância sanitária no Brasil. Cadernos de Saúde Pública. Riode Janeiro: Fiocruz. Recovered from http://www.scielo.br/pdf/csp/v11n1/v11n1a19.pdf
Demari-Silva, B., Vesgueiro, F.T., Sallum, M.A., & Marrelli, M.T. (2011). Taxonomic and phylogenetic relationships between species of the genus Culex (Diptera: Culicidae) from Brazil inferred from the cytochrome c oxidase I mitochondrial gene. Journal of Medical Entomology, 48, 272–279. doi: http://dx.doi.org/10.1603/ME09293
Dibo, M.R., Menezes, R.M.T., Ghirardelli, C.P. Mendonça, A.L., & Chiaravalloti Neto, F. (2011). Presença de culicídeos em município de porte médio do Estado de São Paulo e risco de ocorrência de febre do Nilo Ocidental e outras arboviroses. Revista da Sociedade Brasileira de Medicina Tropical, 44, 496-503. http://dx.doi.org/10.1590/S0037-86822011000400019
Eldridge, B.F. (2005). Mosquitoes, the Culicidae. In: Marquardt WH (ed) Biology of disease vectors. 2nd edn. Chapter 9, Elsevier Academic, Burlington, pp 95-111. 2005. doi: http://dx.doi.org/10.3201/eid1108.050610
Ferreira-de-Brito, A., Ribeiro, I.P., Miranda, R.M., Fernandes, R.S., Campos, S.S., Silva, K.A.B., Castro, M.G., Bonaldo, M.C., Brasil, P., Lourenço-de-Oliveira, R., Ferreira-de-Brito, A., Ribeiro, I.P., Miranda, R.M., Fernandes, R.S., Campos, S.S., Silva, K.A.B., Castro, M.G., Bonaldo, M.C., & Brasil, P. (2016). First detection of natural infection of Aedes aegypti with Zika virus in Brazil and throughout South America. Memórias do Instituto Oswaldo Cruz, 111, 655-658. doi: http://doi.org/10.1590/0074-02760160332
Forattini, O.P. (2002). Culicidologia médica, vol 2: Identificação, Biologia, Epidemiologia. São Paulo - Editora da Universidade de São Paulo.
Gomes, A.C., Natal, D., Paula, M.B., Urbinatti, P.R., Mucci, L.F., & Bitencourt, M.D. (2007). Riqueza e abundância de Culicidae (Diptera) em área impactada, Mato Grosso do Sul, Brasil. Revista de Saúde Pública, 41, 661-664. http://dx.doi.org/10.1590/S0034-89102007000400023
Gutierrez, C.T. (2015). The subgenus Melanoconion of Culex in South America ( Diptera : Culicidae). Universidade de São Paulo - Faculdade de Saúde Pública (Dissertação). 2015.doi: http://dx.doi.org/10.11606/T.6.2015.tde-18052015-102016
Hall, T. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series. http://brownlab.mbio.ncsu.edu/jwb/papers/1999hall1.pdf
Harbach, R.E. (2011). Classification within the cosmopolitan genus Culex (Diptera: Culicidae): The foundation for molecular systematics and phylogenetic research. Acta Tropica, 120, 1-14. doi: http://doi.org/10.1016/j.actatropica.2011.06.005
Harbach, R.E. (2012). Culex pipiens: species versus species complex taxonomic history and perspective. Journal of the American Mosquito Control Association, 28 (4 Suppl.): 10-23. doi: http://doi.org/10.2987/8756-971X-28.4.10
Harbach, R.E. (2013). Mosquito Taxonomic Inventory. Recovered from http://www.mosquito-taxonomic-inventory.info/.
Hebert, P.D.N., Cywinska, A., Ball, S.L., & deWaard, J.R. (2003). Biological identifications through DNA barcodes. Proceedings. Biological sciences / The Royal Society, 270, 313-321. doi: http://doi.org/10.1098/rspb.2002.2218
Hebert, P.D.N., Penton, E.H., Burns, J.M., Janzen, D.H., & Hallwachs, W. (2004). Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences of the United States of America, 101, 14812-14817. doi: https://doi.org/10.1073/pnas.0406166101
Hernández-Triana, L.M., Brugman, V.A., Nikolova, N.I., Ruiz-Arrondo, I., Barrero, E., Thorne, T., Marco, M.F., Krüger, A., Lumley, S., Johnson, N., & Fooks, A.R. (2019). DNA barcoding of British mosquitoes (Diptera, Culicidae) to support species identification, discovery of cryptic genetic diversity and monitoring invasive species. ZooKeys 832, 57-76. doi: https://doi.org/10.3897/zookeys.832.32257
Hoyos-López, R., Suaza-Vasco, J., Rúa-Uribe, G., Uribe, S., & Gallego-Gómez, J.C. (2016). Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean. Memórias do Instituto Oswaldo Cruz, 111, 625-634. doi: http://dx.doi.org/10.1590/0074-02760160096
Hutchings, R.S.G., Sallum, M.A.M., & Hutchings, R.W. (2011). Mosquito (Diptera: Culicidae) diversity of a forest-fragment mosaic in the Amazon rain forest. Journal of Medical Entomology, 48, 173-187. doi: https://dx.doi.org/10.1603/me10061
Kimura, M. (1980). A Simple Method for Estimating Evolutionary Rates of Base Substitutions Through Comparative Studies of Nucleotide Sequences. Journal Molecular Evolution, 16, 111-120. doi: http://dx.doi.org/10.1007/bf01731581
Kobayashi, K.M. (1999). Revisão das espécies do grupo Atratus de Culex (Melanoconion) (Diptera: Culicidae). Tese de Doutorado. Universidade de São Paulo. Faculdade de Saúde Pública.
Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870-1874. http://doi.org/10.1093/molbev/msw054
Lane, J. (1953). Neotropical Culicidae. vol. 2. São Paulo, Press in University of São Paulo.
Laurito, M., Oliveira, T.M.P., Almirón, W.R., & Sallum, M.A.M. (2013). coI barcode versus morphological identification of Culex (Culex) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil. Memórias do Instituto Oswaldo Cruz, 108, 110-122.doi: http://dx.doi.org/10.1590/0074-0276130457
Louise, C., Vidal, P.O., & Suesdek, L. (2015). Microevolution of Aedes aegypti. PLoS ONE 10(9): e0137851. doi: http://dx.doi.org/10.1371/journal.pone.0137851
Pugedo, H., Barata, R.A., França-Silva, J.C., Silva, J.C., & Dias, E.S. (2005). HP: um modelo aprimorado de armadilha luminosa de sucção para a captura de pequenos insetos. Revista da Sociedade Brasileira de Medicina Tropical, 38, 70-72. doi: http://dx.doi.org/10.1590/S0037-86822005000100017
Puillandre, N., Lambert, A., Brouillet, S., & Achaz, G. (2012). ABGD, Automatic Barcode Gap Discovery for primary species delimitation. Molecular Ecology, 21, 1864-1877. doi: http://doi.org/10.1111/j.1365-294X.2011.05239.x
Ratnasingham, S., & Hebert, P.D.N. (2007). BARCODING, BOLD : The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes, 7, 355-364. doi: http://dx.doi.org/10.1111/j.1471-8286.2007.01678.x.
Sallum, M.A.M. (1994). Revisão da Seção Spissipes de Culex (Melanoconion) (Diptera: Culicidae). Recovered from http://www.teses.usp.br/teses/disponiveis/6/6132/tde-15072016-150054/publico/DR_243_Sallum_1994.pdf
Sallum, M.A.M., & Forattini, O.P. (1996). Revision of the Spissipes Section of Culex (Melanoconion) (Dipeta: Culicidae), 12(3):517-600. Recovered from http://www.teses.usp.br/teses/disponiveis/6/6132/tde-15072016 150054/publico/DR_243_Sallum_1994.pdf
Sirivanakarn, S. (1982). A Review of the Systematics and a Proposed Scheme of Internal Classification of the New World Subgenus Melanoconion of Culex. Mosquito Systematics, 14. http://mosquito-taxonomic-inventory.info/sites/mosquito-taxonomic-inventory.info/files/Sirivanakarn%201983.pdf
Song, S., Li, Y., Fu, S., Liu, H., Li, X., Gao, X., Xu, Z., Liu, G., Wang, D., Tian, Z., Zhou, J., He, Y., Lei, W., Wang, H., Wang, B., Lu, X., & Liang, G. (2017). Could Zika virus emerge in Mainland China? Virus isolation from nature in Culex quinquefasciatus, 2016. Emerging Microbes & Infections, 6, e93. doi: http://doi.org/10.1038/emi.2017.80
Sumruayphol, S., Apiwathnasorn, C., Ruangsittichai, J., Sriwichai, P., Attrapadung, S., Samung, Y., & Dujardin, J.P. (2016). DNA barcoding and wing morphometrics to distinguish three Aedes vectors in Thailand. Acta Tropica, 159, 1-10.doi: http://doi.org/10.1016/j.actatropica.2016.03.010
Torres-Gutierrez, C., Bergo, E.S., Emerson, K.J., de Oliveira, T.M.P., Greni, S., Sallum, M.A.M. (2016). Mitochondrial coI gene as a tool in the taxonomy of mosquitoes Culex subgenus Melanoconion. Acta Tropica, 164, 137-149. doi: http://doi.org/10.1016/j.actatropica.2016.09.007
Viana, L.A., Soares, P., Paiva, F., & Lourenço-de-Oliveira, R. (2010). Caiman-Biting Mosquitoes and the Natural Vectors of Hepatozoon caimani in Brazil. Journal of Medical Entomology, 47, 670-676. doi: http://doi.org/10.1603/me09260
Williams, M.R., & Savage, H.M. (2009). Identification of Culex (Melanoconion) species of the United States using female cibarial armature (Diptera: Culicidae). Journal of Medical Entomology, 46, 745-752. doi: http://doi.org/10.1603/033.046.0404
Published
2019-05-27
How to Cite
COSTA DOS SANTOS, Cícera Alexsandra et al.
Identificação de algumas espécies amazônicas de Culex (Culex) e Culex (Melanoconion) por meio de morfotipagem e barcoding.
Acta Brasiliensis, [S.l.], v. 3, n. 2, p. 82-89, may 2019.
ISSN 2526-4338.
Available at: <http://revistas.ufcg.edu.br/actabra/index.php/actabra/article/view/177>. Date accessed: 25 nov. 2024.
doi: https://doi.org/10.22571/2526-4338177.
Section
Genetics
