Identification of some Amazonian species of Culex (Culex) and Culex (Melanoconion) by morphotyping and barcoding

  • Cícera Alexsandra Costa dos Santos Instituto Federal de Educação, Ciência e Tecnologia de Rondônia, Porto Velho, Rondônia, Brasil
  • Lucas Rosendo da Silva Fundação Oswaldo Cruz, Laboratório de Entomologia, Porto Velho, Rondônia, Brasil
  • Anne Caroline Alves Meireles Fundação Oswaldo Cruz, Laboratório de Entomologia, Porto Velho, Rondônia, Brasil
  • Marlon Ferreira Simplício Fundação Oswaldo Cruz, Laboratório de Entomologia, Porto Velho, Rondônia, Brasil
  • Luiz Herman Soares Gil Fundação Oswaldo Cruz, Instituto de Pesquisa em Patologias Tropicais, Porto Velho, Rondônia, Brasil
  • Maisa da Silva Araújo Fundação Oswaldo Cruz, Laboratório de Entomologia, Porto Velho, Rondônia, Brasil
  • Wanne Patrício Soares Fundação Oswaldo Cruz, Laboratório de Entomologia, Porto Velho, Rondônia, Brasil
  • Deusilene Souza Vieira Dall A'cqua Fundação Oswaldo Cruz, Laboratório de Virologia, Porto Velho, Rondônia, Brasil
  • Gabriel Eduardo Melim Ferreira Fundação Oswaldo Cruz, Laboratório de Epidemiologia Genética, Porto Velho, Rondônia, Brasil
  • Genimar Rebouças Julião Fundação Oswaldo Cruz, Laboratório de Entomologia, Porto Velho, Rondônia, Brasil

Resumo

Culex spp. mosquitoes have idiosyncratic characteristics and its low variability makes difficult their identification. The aim of our study was to analyze the 5' region of the cytochrome oxidase subunit I gene (coI) for the taxonomic identification of Culex species which were previously morphotyped and diagnosed in Culex and Melanoconion subgenera at the field conditions. Ten specimen sequences were analyzed by the Automatic Barcode Gap Discovery (ABGD). All sequences showed 94-99% identity when compared to other Culex species sequences available from GenBank. Five initial partitions supported 80-88 species groups. Among them, eight sets contained the specimens of the present study. Of the 10 mosquito sequences, five did not form any consistent cluster, and the remaining showed some consistency in the taxonomic diagnosis at the field conditions. Our results suggest that some coI gene sequences of specimens may belong to species of the subgenus Melanoconion, whose 5’ coI sequence is unknown or unpublished in GenBank.

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Referências

Cardoso, B.F., Serra, O.P., Heinen, L.B.S., Zuchi, N., Souza, V.C., Naveca, F.G., Santos, M.A.M., & Slhessarenko, R.D. (2015). Detection of Oropouche virus segment S in patients and in Culex quinquefasciatus in the state of Mato Grosso, Brazil. Memórias do Instituto Oswaldo Cruz, 110, 745-754. doi: http://dx.doi.org/10.1590/0074-02760150123
Collins, R.A., & Cruickshank, R.H. (2014). Known Knowns, Known Unknowns, Unknown Unknowns and Unknown Knowns in DNA Barcoding: A Comment on Dowton et al. Systematic Biology, 63, 1005-1009. doi: http://doi.org/10.1093/sysbio/syu060
Consoli, R.A.G.B., & Lourenço-de-Oliveira, R. (1994). Principais mosquitos de importância sanitária no Brasil. Cadernos de Saúde Pública. Riode Janeiro: Fiocruz. Recovered from http://www.scielo.br/pdf/csp/v11n1/v11n1a19.pdf
Demari-Silva, B., Vesgueiro, F.T., Sallum, M.A., & Marrelli, M.T. (2011). Taxonomic and phylogenetic relationships between species of the genus Culex (Diptera: Culicidae) from Brazil inferred from the cytochrome c oxidase I mitochondrial gene. Journal of Medical Entomology, 48, 272–279. doi: http://dx.doi.org/10.1603/ME09293
Dibo, M.R., Menezes, R.M.T., Ghirardelli, C.P. Mendonça, A.L., & Chiaravalloti Neto, F. (2011). Presença de culicídeos em município de porte médio do Estado de São Paulo e risco de ocorrência de febre do Nilo Ocidental e outras arboviroses. Revista da Sociedade Brasileira de Medicina Tropical, 44, 496-503. http://dx.doi.org/10.1590/S0037-86822011000400019
Eldridge, B.F. (2005). Mosquitoes, the Culicidae. In: Marquardt WH (ed) Biology of disease vectors. 2nd edn. Chapter 9, Elsevier Academic, Burlington, pp 95-111. 2005. doi: http://dx.doi.org/10.3201/eid1108.050610
Ferreira-de-Brito, A., Ribeiro, I.P., Miranda, R.M., Fernandes, R.S., Campos, S.S., Silva, K.A.B., Castro, M.G., Bonaldo, M.C., Brasil, P., Lourenço-de-Oliveira, R., Ferreira-de-Brito, A., Ribeiro, I.P., Miranda, R.M., Fernandes, R.S., Campos, S.S., Silva, K.A.B., Castro, M.G., Bonaldo, M.C., & Brasil, P. (2016). First detection of natural infection of Aedes aegypti with Zika virus in Brazil and throughout South America. Memórias do Instituto Oswaldo Cruz, 111, 655-658. doi: http://doi.org/10.1590/0074-02760160332
Forattini, O.P. (2002). Culicidologia médica, vol 2: Identificação, Biologia, Epidemiologia. São Paulo - Editora da Universidade de São Paulo.
Gomes, A.C., Natal, D., Paula, M.B., Urbinatti, P.R., Mucci, L.F., & Bitencourt, M.D. (2007). Riqueza e abundância de Culicidae (Diptera) em área impactada, Mato Grosso do Sul, Brasil. Revista de Saúde Pública, 41, 661-664. http://dx.doi.org/10.1590/S0034-89102007000400023
Gutierrez, C.T. (2015). The subgenus Melanoconion of Culex in South America ( Diptera : Culicidae). Universidade de São Paulo - Faculdade de Saúde Pública (Dissertação). 2015.doi: http://dx.doi.org/10.11606/T.6.2015.tde-18052015-102016
Hall, T. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series. http://brownlab.mbio.ncsu.edu/jwb/papers/1999hall1.pdf
Harbach, R.E. (2011). Classification within the cosmopolitan genus Culex (Diptera: Culicidae): The foundation for molecular systematics and phylogenetic research. Acta Tropica, 120, 1-14. doi: http://doi.org/10.1016/j.actatropica.2011.06.005
Harbach, R.E. (2012). Culex pipiens: species versus species complex taxonomic history and perspective. Journal of the American Mosquito Control Association, 28 (4 Suppl.): 10-23. doi: http://doi.org/10.2987/8756-971X-28.4.10
Harbach, R.E. (2013). Mosquito Taxonomic Inventory. Recovered from http://www.mosquito-taxonomic-inventory.info/.
Hebert, P.D.N., Cywinska, A., Ball, S.L., & deWaard, J.R. (2003). Biological identifications through DNA barcodes. Proceedings. Biological sciences / The Royal Society, 270, 313-321. doi: http://doi.org/10.1098/rspb.2002.2218
Hebert, P.D.N., Penton, E.H., Burns, J.M., Janzen, D.H., & Hallwachs, W. (2004). Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences of the United States of America, 101, 14812-14817. doi: https://doi.org/10.1073/pnas.0406166101
Hernández-Triana, L.M., Brugman, V.A., Nikolova, N.I., Ruiz-Arrondo, I., Barrero, E., Thorne, T., Marco, M.F., Krüger, A., Lumley, S., Johnson, N., & Fooks, A.R. (2019). DNA barcoding of British mosquitoes (Diptera, Culicidae) to support species identification, discovery of cryptic genetic diversity and monitoring invasive species. ZooKeys 832, 57-76. doi: https://doi.org/10.3897/zookeys.832.32257
Hoyos-López, R., Suaza-Vasco, J., Rúa-Uribe, G., Uribe, S., & Gallego-Gómez, J.C. (2016). Molecular detection of flaviviruses and alphaviruses in mosquitoes (Diptera: Culicidae) from coastal ecosystems in the Colombian Caribbean. Memórias do Instituto Oswaldo Cruz, 111, 625-634. doi: http://dx.doi.org/10.1590/0074-02760160096
Hutchings, R.S.G., Sallum, M.A.M., & Hutchings, R.W. (2011). Mosquito (Diptera: Culicidae) diversity of a forest-fragment mosaic in the Amazon rain forest. Journal of Medical Entomology, 48, 173-187. doi: https://dx.doi.org/10.1603/me10061
Kimura, M. (1980). A Simple Method for Estimating Evolutionary Rates of Base Substitutions Through Comparative Studies of Nucleotide Sequences. Journal Molecular Evolution, 16, 111-120. doi: http://dx.doi.org/10.1007/bf01731581
Kobayashi, K.M. (1999). Revisão das espécies do grupo Atratus de Culex (Melanoconion) (Diptera: Culicidae). Tese de Doutorado. Universidade de São Paulo. Faculdade de Saúde Pública.
Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870-1874. http://doi.org/10.1093/molbev/msw054
Lane, J. (1953). Neotropical Culicidae. vol. 2. São Paulo, Press in University of São Paulo.
Laurito, M., Oliveira, T.M.P., Almirón, W.R., & Sallum, M.A.M. (2013). coI barcode versus morphological identification of Culex (Culex) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil. Memórias do Instituto Oswaldo Cruz, 108, 110-122.doi: http://dx.doi.org/10.1590/0074-0276130457
Louise, C., Vidal, P.O., & Suesdek, L. (2015). Microevolution of Aedes aegypti. PLoS ONE 10(9): e0137851. doi: http://dx.doi.org/10.1371/journal.pone.0137851
Pugedo, H., Barata, R.A., França-Silva, J.C., Silva, J.C., & Dias, E.S. (2005). HP: um modelo aprimorado de armadilha luminosa de sucção para a captura de pequenos insetos. Revista da Sociedade Brasileira de Medicina Tropical, 38, 70-72. doi: http://dx.doi.org/10.1590/S0037-86822005000100017
Puillandre, N., Lambert, A., Brouillet, S., & Achaz, G. (2012). ABGD, Automatic Barcode Gap Discovery for primary species delimitation. Molecular Ecology, 21, 1864-1877. doi: http://doi.org/10.1111/j.1365-294X.2011.05239.x
Ratnasingham, S., & Hebert, P.D.N. (2007). BARCODING, BOLD : The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes, 7, 355-364. doi: http://dx.doi.org/10.1111/j.1471-8286.2007.01678.x.
Sallum, M.A.M. (1994). Revisão da Seção Spissipes de Culex (Melanoconion) (Diptera: Culicidae). Recovered from http://www.teses.usp.br/teses/disponiveis/6/6132/tde-15072016-150054/publico/DR_243_Sallum_1994.pdf
Sallum, M.A.M., & Forattini, O.P. (1996). Revision of the Spissipes Section of Culex (Melanoconion) (Dipeta: Culicidae), 12(3):517-600. Recovered from http://www.teses.usp.br/teses/disponiveis/6/6132/tde-15072016 150054/publico/DR_243_Sallum_1994.pdf
Sirivanakarn, S. (1982). A Review of the Systematics and a Proposed Scheme of Internal Classification of the New World Subgenus Melanoconion of Culex. Mosquito Systematics, 14. http://mosquito-taxonomic-inventory.info/sites/mosquito-taxonomic-inventory.info/files/Sirivanakarn%201983.pdf
Song, S., Li, Y., Fu, S., Liu, H., Li, X., Gao, X., Xu, Z., Liu, G., Wang, D., Tian, Z., Zhou, J., He, Y., Lei, W., Wang, H., Wang, B., Lu, X., & Liang, G. (2017). Could Zika virus emerge in Mainland China? Virus isolation from nature in Culex quinquefasciatus, 2016. Emerging Microbes & Infections, 6, e93. doi: http://doi.org/10.1038/emi.2017.80
Sumruayphol, S., Apiwathnasorn, C., Ruangsittichai, J., Sriwichai, P., Attrapadung, S., Samung, Y., & Dujardin, J.P. (2016). DNA barcoding and wing morphometrics to distinguish three Aedes vectors in Thailand. Acta Tropica, 159, 1-10.doi: http://doi.org/10.1016/j.actatropica.2016.03.010
Torres-Gutierrez, C., Bergo, E.S., Emerson, K.J., de Oliveira, T.M.P., Greni, S., Sallum, M.A.M. (2016). Mitochondrial coI gene as a tool in the taxonomy of mosquitoes Culex subgenus Melanoconion. Acta Tropica, 164, 137-149. doi: http://doi.org/10.1016/j.actatropica.2016.09.007
Viana, L.A., Soares, P., Paiva, F., & Lourenço-de-Oliveira, R. (2010). Caiman-Biting Mosquitoes and the Natural Vectors of Hepatozoon caimani in Brazil. Journal of Medical Entomology, 47, 670-676. doi: http://doi.org/10.1603/me09260
Williams, M.R., & Savage, H.M. (2009). Identification of Culex (Melanoconion) species of the United States using female cibarial armature (Diptera: Culicidae). Journal of Medical Entomology, 46, 745-752. doi: http://doi.org/10.1603/033.046.0404
Publicado
2019-05-27
Como Citar
COSTA DOS SANTOS, Cícera Alexsandra et al. Identification of some Amazonian species of Culex (Culex) and Culex (Melanoconion) by morphotyping and barcoding. Acta Brasiliensis, [S.l.], v. 3, n. 2, p. 82-89, maio 2019. ISSN 2526-4338. Disponível em: <http://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/177>. Acesso em: 17 jul. 2019. doi: https://doi.org/10.22571/2526-4338177.
Seção
Genética