Effect of Bacillus sp. and Trichoderma sp. in Micelial Growth of Sclerotium rolfsii

Abstract

This research aimed to evaluate the antagonistic effect of bacterial isolates of the genus Bacillus sp. and the fungus Trichoderma sp. on the mycelial growth of Sclerotium rolfsii, causal agent of sclerotia rot in plants Vigna unguiculata (L.) Walp. (cowpea). The assay was conducted using the crop pairing technique. The pathogen was obtained by the isolation of V. unguiculata (L.) Walp. with symptoms of the disease. The antagonists used were: Bacillus subtilis (LCB 30), B. subtilis (LCB 45), Bacillus sp. (BMH), Bacillus sp. (INV) and Trichoderma sp. Evaluations of mycelial growth were made when the entire surface of the culture medium was presented BDA-colonized by S. rolfsii in the treatment control (pathogen grown in the absence of antagonist). For the analyzes, a completely randomized design with five replications was used. The isolates B. subtilis (LCB30), Bacillus sp. (INV) and Trichoderma sp. They are capable of inhibiting fungus mycelial growth of S. rolfsii by reducing the fungal colony on 53.77%, 29.22% and 34.00%, respectively, with isolated B. subtilis (LCB45) and Bacillus sp. (BMH), inhibited 13.55% and 21.55% growth S.rolfsii, respectively, did not differ statistically from the control.

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References

Araújo, F.F. de. & Marchesi, G.V.P. (2009). Uso de Bacillus subtilis no controle da meloidoginose e na promoção do crescimento do tomateiro. Ciência Rural, 39(5), 1558-1561. doi: http://dx.doi.org/10.1590/S0103-84782009000500039

Assunção, I.P., Nascimento L.D., Ferreira M.F., Oliveira F.J., Michereff S. J. & Lima G.S.A. (2011). Reaction of faba bean genotypes to Rhizoctonia solani and resistance stability. Horticultura Brasileira, 29(4), 492-497. doi: http://dx.doi.org/10.1590/S0102-05362011000400008

Auler, A.C.V., Carvalho, D.D.C. & Mello, S.C.M. (2013). Antagonismo de Trichoderma harzanium a Sclerotium rolfsii nas culturas do feijoeiro e soja. Revista Agro@mbiente On-line, 7(3), 359-365. doi: http://dx.doi.org/10.18227/1982-8470ragro.v7i3.1335

Bontempo, F.A. (2016). Seleção “in vitro” de isolados de Trichoderma spp. E Bacillus spp. em baixa temperatura de crescimento para o controle de Sclerotium cepivorum. Dissertação (Pós-Graduação). Universidade Federal de Viçosa – Campus Rio Parnaíba, MG. Brasil. Recuperado de http://www.locus.ufv.br/handle/123456789/7805

Campos, J.T. de. (2010). Rizobactérias promotoras do crescimento de cana-de-açúcar. Dissertação (Mestrado). Instituto Agronômico de Campinas, Campinas, SP, Brasil.

Companhia Nacional de Abastecimento (CONAB) (2017). Acompanhamento da safra brasileira de grãos, safra 2016/17. Recuperado de https://www.conab.gov.br/info-agro/safras/graos/boletim-da-safra-de-graos/item/download/21088_8ca248b277426bb3974f74efa00abab6

Coutinho, P.W.R., Silva, de D.M.S., Saldanha, E.C.M., Okumura, R.S. & Silva Júnior, M.L. da. (2014). Doses de fósforo na cultura do feijão-caupi na região nordeste do estado do Pará. RevistaAgro@mbiente On-line, 8(1), 66-73. doi: http://dx.doi.org/10.18227/1982-8470ragro.v8i1.1310

Dennis, C. & Webster, J. (1971). Antagonistic properties of species-groups of Trichoderma. III. Hyphal interactions. Transactions British Mycological Society, 57, 363-369. doi: https://doi.org/10.1016/S0007-1536(71)80050-5

Ferreira, D.F. (2010). SISVAR: Sistema de análise de variância. Universidade Federal de Lavras, (CD-ROM).838.

Isaias, C.O., Martins, I., Silva, J.B.T., Silva, J.P. & Mello, S.C.M. (2014). Ação antagônica e de metabólitos bioativos de Trichoderma spp. contra os patógenos Sclerotium rolfsii e Verticillium dahliae. Summa Phytopathologica, 40(1), 34-41. doi: http://dx.doi.org/10.1590/S0100-54052014000100005

Lanna Filho, R., Ferro, H.M. & de Pinho, R.S.C. (2010). Controle biológico mediado por Bacillus subtilis. Revista Trópica: Ciências Agrárias e Biológicas, 4(2), 12-20. http://www.periodicoseletronicos.ufma.br/index.php/ccaatropica/article/view/145

Maciel, C.G., MacielI, C.G., Lazarotto, M., Ricardo Mezzomo, R., Poletto, I., Muniz, M.F.B. & Lippert, D.B. (2012). Trichoderma spp. no biocontrole de Cylindrocladium candelabrum em mudas de Eucalyptus saligna. Revista Árvore, 36(5), 825-832. doi: http://dx.doi.org/10.1590/S0100-67622012000500004

Mariano, R.L.R., Silveira, E.B. & Gomes, A.M.A. (2005). Controle biológico de doenças radiculares. Em: Michereff, S.J., Andrade, D.E.G.T. e Menezes, M. Ecologia e manejo de patógenos radiculares em solos tropicais. Impresa Universitária, UFRPE, Recife, 303-320.

Morsy, E.M., Abdel-Kawi, K.A. & Khalil, M.N.A. (2009). Efficiency of Trichoderma viride and Bacillus subtilis as biocontrol agents against Fusarium solani on tomato plants. Egyptian Journal of Phytopathology, 37, 47-57.

Remuska, A.C. & Pria, M.D. (2007). Efeito de Bacillus thuringiensis e Trichoderma sp. no crescimento de fungos fitopatogênicos. UEPG Ci. Exatas Terra, Ci. Agr. Eng., Ponta Grossa, 13(3), 31-36. http://ri.uepg.br/riuepg/handle/123456789/125

Silva, J.A., Oliveira, M.G., Souza, L.T., Assunção, I.P., Lima, G.A. & Michereff, S.J. (2014). Reação de genótipos de feijão-fava a Sclerotium rolfsii. Horticultura Brasileira, Vol. 32, pp. 98-101. doi: http://dx.doi.org/10.1590/S0102-05362014000100016

Singh, U.P. e Sarma, B.K. (2009). Biology and control of Sclerotium rolfsii: the incitant of colar rot of Cicer arietinum. Saarbrucken: VDM, 198p.

Sousa, T.G. de. e Blum, L.E.B. (2013). Uso de Trichoderma harzianum e condicionador orgânico de solo para controle da podridão por Sclerotium rolfsii. Bioscience Journal, 29(5), 1616-1623. http://www.seer.ufu.br/index.php/biosciencejournal/article/view/13932
Published
2019-05-27
How to Cite
FERREIRA DE SÁ, Mylenna Nádja et al. Effect of Bacillus sp. and Trichoderma sp. in Micelial Growth of Sclerotium rolfsii. Acta Brasiliensis, [S.l.], v. 3, n. 2, p. 79-81, may 2019. ISSN 2526-4338. Available at: <http://revistas.ufcg.edu.br/actabra/index.php/actabra/article/view/158>. Date accessed: 09 nov. 2024. doi: https://doi.org/10.22571/2526-4338158.
Section
Microbiology