In vitro selection of biocontrol agents for the control of Fusarium sp.

Abstract

The objective of this research was to evaluate the in vitro antagonistic action of Bacillus and Trichoderma sp. in the control of Fusarium sp. responsible for damages in plants of Vigna unguiculata (L.) Walp (cowpea). The assay was conducted using the crop pairing technique. The pathogen Fusarium sp. was obtained by the isolation of cowpea plants with symptoms of the disease. The antagonists used were: Bacillus subtilis (LCB 30), B. subtilis (LCB 45), Bacillus sp. (BMH), Bacillus sp. (INV) and Trichoderma sp. Mycelial growth evaluations were performed when the entire surface of the PDA (Potato Dextrose Agar) culture medium was colonized by Fusarium sp. in the control treatment (pathogen cultured in the absence of the antagonist). For the analyzes, a completely randomized design with 5 replications was used. From the results obtained, it can be inferred that the isolates of Bacillus spp. and Trichoderma sp. have the potential as antagonists against Fusarium sp., the isolate Bacillus subtilis (LCB 45) responsible for inhibiting 25% of the mycelial growth, and the treatments with B. subtilis (LCB 30), Bacillus sp. (BMH), Bacillus sp. (INV) and Trichoderma sp., Inhibited 59.4%, 46.5%, 46.4% and 37% growth of the colony diameter of the causal agent of fusariosis in cowpea plants, respectively.

Downloads

Download data is not yet available.

References

Araújo, L. B. R. (2017). Potencial genético de variedades tradicionais de feijão-caupi e avaliação para resistência à murcha de Fusarium (Dissertação de mestrado). Universidade Federal do Ceará, Fortaleza, Ceará, Brasil. Recuperado de http://www.repositorio.ufc.br/handle/riufc/25240
Bettiol, W. & Ghini, R. Controle biológico. In A. Bergamin, H. Kimati & L. Amorim. (1995). Manual de fitopatologia.: Princípios e conceitos (3 ed., vol. 1, pp. 717-727) São Paulo: Agronômica Ceres.
Bettiol, W. & Morandi, M. A. B. (2009). Biocontrole de doenças de plantas: uso e perspectivas. Jaguariuna: Embrapa Meio Ambiente.
Bomfim, M. P., São José, A. R., Rebouças, T. N. H., Almeida, S. S., Souza, I. V. B. & Dias, N. O. (2010). Avaliação antagônica in vitro e in vivo de Trichoderma spp. a Rhizopus stolonifer em maracujazeiro amarelo. Summa Phytopathologica, 36(1), 61-67. doi: 10.1590/S0100-54052010000100011
Carvalho, D. D. C., Mello, S. C. M., Lobo Júnior, M. & Silva, M. C. (2011). Controle de Fusarium oxysporum f.sp. phaseoli in vitro e em sementes, e promoção do crescimento inicial do feijoeiro comum por Trichoderma harzianum. Tropical Plant Pathology, 36(1), 28-34. doi: 10.1590/S1982-56762011000100004
Brígida Dean, R., Van Kan, J. A. L., Pretorius, Z. A., Hammond-Kosack, K. E., Di Pietro, A., Spanu, P. D., ...Foster, G. D. (2012). The top 10 fungal pathogens in molecular plant pathology. Molecular Plant Pathology, 13(4), 414-430. doi: 10.1111/j.1364-3703.2011.00783.x
Dubey, S. C., Suresh, M. & Singh, B. (2007). Evaluation of Trichoderma species against Fusarium oxysporum f.sp. ciceris for integrated management of chickpea wilt. Biological Control, 40(1), 118-127. doi: 10.1016/j.biocontrol.2006.06.006
Dennis C.;,Webster J. (1971). Antagonistic properties of species groups of Trichoderma, III Hyphal interactions. Transactions British Mycological Society, 57(3), 363-369. doi: 10.1016/S0007-1536(71)80050-5
Ferreira, D. F. (2010). SISVAR: Sistema de análise de variância. Universidade Federal de Lavras. (CD-ROM).
Kupper, K. C., Gimenes-Fernandes, N. & Goes, A. (2003). Controle biológico de Colletotrichum acutatum, agente causal da queda prematura dos frutos cítricos. Fitopatologia Brasileira, 28(3), 251-257. doi: 10.1590/S0100-41582003000300005
Lanna Filho, R., Ferro, H. M. & Pinho, R. S. C. (2010). Controle biológico mediado por Bacillus subtilis. Revista Trópica: Ciências Agrárias e Biológicas, 4(2), 12-20.
Lopes, F. A. C., Steindorff, A. S., Geraldine, A. M., Brandao, R. S., Monteiro, V. N., Lobo Junior, M., …Silva, R. N. (2012). Biochemical and metabolic profiles of Trichoderma strains isolated from common bean crops in the Brazilian Cerrado, and potential antagonism against Sclerotinia sclerotiorum. Fungal Biology, 116(7), 815-824. doi: 10.1016/j.funbio.2012.04.015
Nechet, K. L. & Halfeld-Vieira, B. A. (2006). Doenças do feijão caupi em Roraima. Boa Vista: Embrapa, Roraima.
Raut, J.S. e Karuppayil, S.M. (2014). A status review on the medicinal properties of essential oils. Industrial Crops and Prodcuts, 62, 250-264. doi: 10.1016/j.indcrop.2014.05.055
Santos, D., Corlett, F. M. F., Mendes, J. E. M. F. & Wanderley, J. S. A. (2002). Produtividade e morfologia de vagens e sementes de variedades de fava no Estado da Paraíba. Pesquisa Agropecuária Brasileira, 37(10), 1407-1412. doi: 10.1590/S0100-204X2002001000008
Silva, G. B. P., Heckler, L. I., Santos, R. F., Durigon, M. R. & Blume, E. (2015). Identificação e utilização de Trichoderma spp. armazenados e nativos no biocontrole de Sclerotinia sclerotiorum. Revista Caatinga, 28(4), 33-42. doi: 10.1590/1983-21252015v28n404rc
Soares, P. L. M. (2006). Estudo do controle biológico de fitonematóides com fungos nematófagos (Tese de doutorado). Universidade Estadual Paulista, Jaboticabal, São Paulo, Brasil. Recuperado de https://repositorio.unesp.br/handle/11449/102317
Li, Y., Hu, C. & Yu, Y. 2008. Interfacial studies of sisal fiber reinforced high density polyethylene (HDPE) composites. Composites Part A: Applied Science and Manufacturing, 39(4): 570-578. doi: 10.1016/j.compositesa.2007.07.005
Mohammed, R.R.; Chong, M.F., 2014. Treatment and decolorization of biologically treated palm oil mill effluent (POME) using banana peel as novel biosorbent. Journal of Environmental Management, 132, 237-249. doi: 10.1016/j.jenvman.2013.11.031
Moriwaki, H., Kitajima, S. & Kurashima, M. 2009. Utilization of silkworm cocoon waste as a sorbent for the removal of oil from water. Journal of Hazardous Materials, 165 (1-3): 266–270. doi: 10.1016/j.jhazmat.2008.09.116
Parab, H., Joshi, S., Sudersanan, M., Shenoy, N., Lali, A. & Sarma, U. 2010. Removal and recovery of cobalt from aqueous solutions by adsorption using low cost lignocellulosic biomass-coir pith. Journal of Environmental Science and Health, Part A, Toxic/Hazardous Substances and Environmental Engineering, 45(5): 603-611. doi: 10.1080/10934521003595662
Vinale, F., Sivasithamparam. K., Ghisalberti, E. L., Marra, R., Woo, S. L. & Lorito, M. (2008). Trichoderma-plant-pathogen interactions. Soil Biology & Biochemistry, 40(1), 1-10. doi: 10.1016/j.soilbio.2007.07.002
Published
2019-01-02
How to Cite
FERREIRA DE SÁ, Mylenna Nádja et al. In vitro selection of biocontrol agents for the control of Fusarium sp.. Acta Brasiliensis, [S.l.], v. 3, n. 1, p. 14-16, jan. 2019. ISSN 2526-4338. Available at: <http://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/157>. Date accessed: 05 aug. 2020. doi: https://doi.org/10.22571/2526-4338157.
Section
Microbiology

Most read articles by the same author(s)